Journal: Life Metabolism

Article Title: Regulation of hepatic lipid metabolism by intestine epithelium-derived exosomes

doi: 10.1093/lifemeta/load044

Figure Lengend Snippet: miR-21a-5p and miR-145a-5p regulate liver lipid metabolism through direct suppression of Ccl1 and Btg1 , respectively. (a–e) CCL1-dependent mechanism by which miR-21a-5p regulates liver lipid metabolism. (a) Dual-luciferase assay was performed to validate the direct interaction between miR-21a-5p and 3ʹ-UTR of Ccl1 gene. (b) Raw264.7 cells were transfected with miR-21a-5p mimics or scramble sequences, and the mRNA levels of inflammation marker genes were analyzed by RT-qPCR. (c and d) Representative F4/80 staining of liver section and numbers of F4/80 + cells in HFD-induced obese mice injected with AAV9-miR-21a-5p or AAV-9 scramble. (e) Cellular TG and cholesterol in AML12 cells co-cultured with miR-21a-5p-treated Raw264.7 cells. Results were expressed as mean ± SEM and analyzed by one-way or two-way ANOVA, or Student’s t -test, n = 5 − 7, * denotes P < 0.05. (f–k) MiR-145a-5p regulates liver lipid metabolism through direct suppression of Btg1 . (f) Dual-luciferase assay was performed to validate the direct interaction between miR-145a-5p and 3ʹ-UTR of Btg1 gene. (g) TG concentration of AML12 cells transfected with miR-145a-5p mimics and a plasmid expressing Btg1 -encoding sequences without 3ʹ-UTR. Protein expression of SCD1 in livers of mice treated with AAV9-miR-145a-5p-sponge (h and i) or intExo/miR-145a-5p (j and k). Results were expressed as mean ± SEM and analyzed by one-way ANOVA or Student’s t -test, n = 4 − 8, * denotes P < 0.05.

Article Snippet: SCD1 Rabbit pAb , ABclonal , A16429.

Techniques: Luciferase, Transfection, Marker, Quantitative RT-PCR, Staining, Injection, Cell Culture, Concentration Assay, Plasmid Preparation, Expressing

Journal: Life Metabolism

Article Title: Regulation of hepatic lipid metabolism by intestine epithelium-derived exosomes

doi: 10.1093/lifemeta/load044

Figure Lengend Snippet: qPCR primers.

Article Snippet: SCD1 Rabbit pAb , ABclonal , A16429.

Techniques:

Journal: Life Metabolism

Article Title: Regulation of hepatic lipid metabolism by intestine epithelium-derived exosomes

doi: 10.1093/lifemeta/load044

Figure Lengend Snippet:

Article Snippet: SCD1 Rabbit pAb , ABclonal , A16429.

Techniques:

Journal: The Journal of toxicological sciences

Article Title: Fish oil feeding reverses hepatomegaly and disrupted hepatic function due to the lack of FXR signaling.

doi: 10.2131/jts.42.671

Figure Lengend Snippet: Fig. 4. Infl uence of fi sh oil feeding on hepatic Fas and Scd1 protein levels of S9 fractions in Fxr-null and wild-type mice. A) Fas: fatty acid synthase, B) Scd1: stearoyl CoA desaturase 1. Values are presented as mean ± S.D. (n = 6). Signifi cant differences (*p < 0.05; **p < 0.01) were assessed by the Tukey-Kramer test. Cont, control group; Fish, fi sh oil group; Wild-type, wild- type mice; Fxr-null, Fxr-null mice.

Article Snippet: Goat anti-Scd1 antibody (S-15: Santa Cruz Biotechnology, Dallas, TX, USA), rabbit anti-Fas antibody (H300: Santa Cruz Biotechnology), rabbit anti-Cpt2 antibody (EPR13626: Abcam, Cambridge, UK), rabbit anti-Cpt1A antibody (15184-1-AP: Proteintech Group Inc., Chicago, IL, USA), rabbit anti-Cox IV antibody (11242-1-AP: Proteintech Group Inc.) and monoclonal anti-β-actin anti- body (AC-15: Sigma-Aldrich, St. Louis, MO, USA) were used as primary antibodies.

Techniques: Control